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Next-Level Biomolecule Characterisation

For over 50 years, Applied Photophysics has delivered precision spectroscopy solutions for cutting-edge research and biopharmaceutical development.

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Nicoya Acquires Applied Photophysics – Expanding biologics characterization capabilities for faster time-to-clinic and reduced late-stage failures.

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Pioneering Biophysical Characterisation Since 1970

Applied Photophysics of Leatherhead, Surrey, UK, is a leading provider of solutions for the biophysical characterisation of biomolecules. With over five decades of innovation, we have been at the forefront of spectroscopy for life science research.

Our Chirascan™ systems use circular dichroism (CD) to characterise changes in the higher-order structure of proteins—critical for innovator drugs and biosimilars. The SX range of stopped-flow spectrometers is the global gold standard for kinetic studies of fast biochemical reactions.

Through a joint venture with Fluorescence Innovations Inc., operating as Protein Stable, we offer high-throughput protein stability screening with microplate-based intrinsic fluorescence thermal ramping and chemical melt plate readers.

50+ Years of Innovation Global Scientific Leadership Trusted by Top Pharma
Chirascan CD Systems

Our Product Range

Discover the instruments trusted by thousands of researchers and biopharma professionals worldwide.

Chirascan CD System

Chirascan™ CD Systems

High-performance circular dichroism spectrometers for protein secondary and tertiary structure analysis. Ideal for biosimilar development and conformational stability studies.

  • • Far-UV and near-UV CD measurements
  • • Temperature-controlled sample compartments
  • • Automation-ready for high-throughput workflows
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SX Stopped-Flow Spectrometer

SX Stopped-Flow Spectrometers

The gold standard for rapid kinetic measurements. Capture millisecond-scale biochemical reactions with unmatched precision and reproducibility.

  • • Dead times as low as 0.3 ms
  • • Multi-mixing and quench-flow options
  • • Compatible with UV-Vis, fluorescence, CD detection
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nano-DSF Platform

nano-DSF Platforms

Label-free, microvolume protein stability screening using intrinsic fluorescence. High-throughput thermal and chemical denaturation analysis.

  • • No dyes or labels required
  • • 96- and 384-well plate formats
  • • Low sample consumption (2–10 µL)
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Chirascan V100

Chirascan V100

Advanced CD spectrometer with extended wavelength range and enhanced sensitivity for detailed structural analysis of proteins and nucleic acids.

  • • 170–900 nm wavelength range
  • • High photon flux for low-concentration samples
  • • Integrated Peltier temperature control
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SX20 Stopped-Flow

SX20 Stopped-Flow

Versatile kinetic platform for studying enzyme mechanisms, protein folding, and ligand binding with multi-mixing and sequential injection capabilities.

  • • Up to 4 mixing stages
  • • Quench-flow and freeze-quench options
  • • Real-time data acquisition and analysis
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Protein Stability Screening

Protein Stability Screening

Automated platforms for high-throughput stability profiling of proteins and AAVs. Ideal for formulation development and gene therapy programs.

  • • Integration with liquid handlers
  • • Cloud-based data management
  • • Pre-configured assay templates
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Chirascan Q100

Chirascan Q100

Compact CD spectrometer designed for routine analysis and QC environments. Offers excellent performance with minimal footprint.

  • • Benchtop design
  • • User-friendly software interface
  • • Ideal for biosimilar comparability studies
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SX60 Stopped-Flow

SX60 Stopped-Flow

High-end stopped-flow system for advanced kinetic research. Features precision syringes, low noise detection, and modular configuration.

  • • Sub-millisecond dead time
  • • Multi-wavelength detection
  • • Optional CD and fluorescence modules
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Applications in Research & Industry

CD Applications

Study protein folding, secondary structure changes, ligand binding, and conformational stability in response to pH, temperature, or denaturants.

Stopped-Flow Applications

Investigate fast enzyme kinetics, protein folding dynamics, electron transfer, and transient intermediate formation in real time.

Differential Scanning Fluorimetry

Perform high-throughput protein stability screening, formulation optimization, and AAV serotype characterisation for gene therapy.

Service & Support

Documents & Software

Access user manuals, software downloads, and application notes for all instruments.

Support Plans

Comprehensive maintenance and support packages tailored to your needs.

Upgrades

Enhance your system with the latest detectors, automation, or software features.

Training

On-site or remote training for operators and advanced users.

50+
Years of Innovation
1000+
Customers Worldwide
40+
Countries Served
98%
Customer Satisfaction

Frequently Asked Questions

What is circular dichroism (CD) spectroscopy?

CD spectroscopy measures differences in the absorption of left and right circularly polarized light. It is used to study the secondary and tertiary structure of proteins and nucleic acids.

How does stopped-flow work?

Stopped-flow rapidly mixes small volumes of reactants and stops the flow abruptly, allowing the observation of fast reactions on millisecond timescales using various detection methods.

What are the advantages of nano-DSF?

nano-DSF requires no dyes or labels, uses minimal sample volume (2–10 µL), and enables high-throughput screening of protein stability under various conditions.

Do you offer international support?

Yes, Applied Photophysics has service engineers and distributors in over 40 countries, including direct offices in the UK, US, and China.

Get in Touch

Have questions about our products or services? Visit our official website to contact the team.

Contact Information

Sales Enquiries

Email: Sales@Photophysics.Com

Phone: +44 1372 386 537 (UK), +1 978 473 7477 (US)

Technical Support

Email: Support@Photophysics.Com

Phone: +44 1372 386 537

Headquarters (UK)

Applied Photophysics Limited

21 Mole Business Park

Leatherhead, Surrey KT22 7BA

United Kingdom

US Office

Applied Photophysics Inc

2331 Crownpoint Executive Drive, Suite F

Charlotte, NC 28227, USA

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